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Journal: MicrobiologyOpen
Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine
doi: 10.1002/mbo3.1169
Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay
Article Snippet:
Techniques: Multiplex Assay
Journal: MicrobiologyOpen
Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine
doi: 10.1002/mbo3.1169
Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP
Article Snippet:
Techniques: Amplification, Control